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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Treinta y Tres. |
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Fecha : |
22/07/2018 |
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Actualizado : |
28/11/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
WEINROTH, M.D.; SCOTT, H.M.; NORBY, B.; LONERAGAN, G.H.; NOYES, N.R.; ROVIRA, P.J.; DOSTER, E.; YANG, X.; WOERNER, D.R.; MORLEY, P.S.; BELK, K.E. |
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Afiliación : |
M.D. WEINROTH, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program); H.M. SCOTT, Universidad de Texas A&M; B. NORBY, Universidad de Michigan State (MSU); G.H. LONERAGAN, Texas Tech University; N.R. NOYES, Universidad de Colorado State (CSU); PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. DOSTER, Universidad de Colorado State (CSU); X. YANG, Universidad de California Davis (UCD); D.R. WOERNER, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program); P. S. MORLEY, Universidad de Colorado State (CSU); K. E. BELK, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program). |
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Título : |
Effects of ceftiofur and chlortetracycline on the resistomes of feedlot cattle. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Applied and Environmental Microbiology, July 2018, vol. 84, no.13, e00610-18. |
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Descripción física : |
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
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ISSN : |
0099-2240 |
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DOI : |
10.1128/AEM.00610-18 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 15 March 2018. / Accepted 24 April 2018. / Accepted manuscript posted online 4 May 2018. OPEN ACCESS. |
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Contenido : |
ABSTRACT.
Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, genes that were selectively enriched through culture and qPCR were not identified through shotgun metagenomic sequencing in this study, suggesting that changes previously documented did not reflect changes affecting the majority of bacterial genetic elements found in the predominant fecal resistome.
© 2018 American Society for Microbiology. MenosABSTRACT.
Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, gen... Presentar Todo |
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Palabras claves : |
ANTIBIOTIC RESISTANCE; ANTIMICROBIAL AGENTS; CATTLE; METAGENOMICS; POSTANTIBIOTIC EFFECT. |
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Thesagro : |
FEEDLOT. |
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Asunto categoría : |
--
L02 Alimentación animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12027/1/e00610-18.full.pdf
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Marc : |
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001 1058828
005 2018-11-28
008 2018 bl uuuu u00u1 u #d
022 $a0099-2240
024 7 $a10.1128/AEM.00610-18$2DOI
100 1 $aWEINROTH, M.D.
245 $aEffects of ceftiofur and chlortetracycline on the resistomes of feedlot cattle.$h[electronic resource]
260 $c2018
300 $cThis is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
500 $aArticle history: Received 15 March 2018. / Accepted 24 April 2018. / Accepted manuscript posted online 4 May 2018. OPEN ACCESS.
520 $aABSTRACT. Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, genes that were selectively enriched through culture and qPCR were not identified through shotgun metagenomic sequencing in this study, suggesting that changes previously documented did not reflect changes affecting the majority of bacterial genetic elements found in the predominant fecal resistome. © 2018 American Society for Microbiology.
650 $aFEEDLOT
653 $aANTIBIOTIC RESISTANCE
653 $aANTIMICROBIAL AGENTS
653 $aCATTLE
653 $aMETAGENOMICS
653 $aPOSTANTIBIOTIC EFFECT
700 1 $aSCOTT, H.M.
700 1 $aNORBY, B.
700 1 $aLONERAGAN, G.H.
700 1 $aNOYES, N.R.
700 1 $aROVIRA, P.J.
700 1 $aDOSTER, E.
700 1 $aYANG, X.
700 1 $aWOERNER, D.R.
700 1 $aMORLEY, P.S.
700 1 $aBELK, K.E.
773 $tApplied and Environmental Microbiology, July 2018, vol. 84, no.13, e00610-18.
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INIA Las Brujas (LB) |
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| Registros recuperados : 72 | |
| 1. |  | GARCÍA, S. Control de enfermedades en cebolla dulce. ln: INIA Las Brujas. Programa Horticultura. Transplante de cebolla dulce. Jornada técnica. Canelones (UY): INIA Las Brujas, 1994. p. 14-22 (INIA Serie Actividades de Difusión ; 12) Participan: INIA Las Brujas, CALMER, UTU Libertad, Club Reformers.| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 7. | | García, S. Enfermedades en cebolla Las Brujas, Canelones (Uruguay): INIA, 1995. p. 151-168 (INIA Serie Actividades de Difusión ; 46) INIA Las Brujas| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 9. | | GARCÍA, S. Manejo de enfermedades. ln: INIA (Instituto Nacional de Investigación Agropecuaria). Programa Fruticultura. Tecnología disponible para la producción de durazneros en la zona norte del país. Jornada de divulgación (Canelones, 9 junio 1994). Canelones (UY): INIA Las Brujas, 1994. p. 17-24 (INIA Serie Actividades de Difusión ; 16)| Tipo: Actividades de Difusión |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| Registros recuperados : 72 | |
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